TABLE 1.
Oligonucleotide primers for amplification of E. chaffeensis Ank200 and TRP47 gene fragments
| Fragment | Sequence (5′ to 3′) of indicated primer |
Size (bp) | |
|---|---|---|---|
| Forward primera | Reverse primer | ||
| Ank200 | |||
| N | CAACAAAATCCTAATTCGCAAG | CGATTTTATATCATTACCAGCA | 1,644 |
| N1 | CACCATGGCAGATCCAAAACAAG | TACCGCATACAATGGATCTTC | 384 |
| N2 | CACCCCTTTACCTAAAGGTCAAAG | ATCCCTAACACCTTCCC | 456 |
| N3 | CACCGCAGTTATTCATGATGAAGAG | CAATGGGGATTGATTTC | 468 |
| N4 | CACCCATGTTATGGTTCAGAACC | ATCATTACCAGCAACAGC | 354 |
| N5 | CACCATGGCAGATCCAAAACAAG | TTGCTGAGAAGGCAAATC | 195 |
| N6 | CACCGAAACAGGAGAAACTGTAGAA | TACCGCATACAATGGATCTTC | 189 |
| N7 | CACCGCAGTTATTCATGATGAAGAG | AGCTAAATGCAGTAATGTCATTAC | 246 |
| N8 | CACCGTAATGACATTACTGCATTTAGCT | CAATGGGGATTGATTTC | 246 |
| N9 | CACCGCAGTTATTCATGATGAAGAG | AATTTCTTCTAGATCTGGCTC | 123 |
| N10 | CACCGAGCCAGATCTAGAAGAAATT | AGCTAAATGCAGTAATGTCATTAC | 144 |
| I | TGTTCAGTTAAAGGACGTGTTC | AGCTAAATGCAGCGGTGTATC | 1,371 |
| C | TTTGCTGAAAAGGGTGTAAAAA | ATCTTCAGATGTAATAGGAGGTAGTCCC | 1,368 |
| C1 | TTTGCTGAAAAGGGTGTAAAAA | TCCATGTAGACCATGAACTGC | 822 |
| C2 | GCAGTTCATGGTCTACATGGA | TTTGCTCTGGCAAGAACTT | 639 |
| C3 | GCAGTTCATGGTCTACATGGA | CGCTGATGCACCTAGAGA | 318 |
| C4 | TCTCTAGGTGCATCAGCG | TTTGCTCTGGCAAGAACTT | 339 |
| C5 | TCTCTAGGTGCATCAGCG | ACCCTTATCAAATATTCCACT | 171 |
| C6 | AGTGGAATATTTGATAAGGGT | TTTGCTCTGGCAAGAACTT | 189 |
| TRP47 | |||
| N1 | ATGCTTCATTTAACAACAGAA | ATGATAACCACGATCAGGTTC | 135 |
| N2 | GAACCTGATCGTGGTTATCAT | AGGATCAACTAAGAAAGAAGC | 135 |
| N3 | GCTTCTTTCTTAGTTGATCCT | ATGATCATGTTCATTGTGATG | 132 |
| N4 | CATCACAATGAACATGATCATG | ATTTCCTTCAAGAACTGGAAC | 132 |
a
Linker sequences for cloning are underlined.