FIG. 5.
Relative fold differences in levels of cytokine mRNA expression following PPDj stimulation for IFN-γ (A), IL-4 (B), and IL-10 (C). All mRNA values are normalized to the value for β2-microglobulin mRNA and are compared to the reference value. The mean PCR amplification efficiencies were 2.01, 2.00, 1.99, and 2.00 for β2-microglobulin, IFN-γ, IL-4, and IL-10, respectively. *, P < 0.05 for significant changes after antigen stimulation. The results are presented as the means ± standard errors of the means.