Mean levels of cytokine mRNA for IFN-γ (A), IL-4 (B), and IL-10 (C) detected in circulating blood samples normalized to the value for β2-microglobulin and compared to the reference values. The mean PCR amplification efficiencies were 1.99, 2.00, 1.96, and 1.98 for β2-microglobulin, IFN-γ, IL-4, and IL-10, respectively. *, P < 0.05 for IL-4 mRNA values in the asymptomatic and MB groups that were significantly different from those for the controls. The results are presented as the means ± standard deviations.