Table 1.
Hemodynamics, hematocrit, Fulton's Index, PHE contraction, and ACh and SNP relaxation in pulmonary and mesenteric arteries of normoxic, hypoxic, and MCT-treated rats
| Parameter | Control | Hypoxia | MCT-Treated |
|---|---|---|---|
| RVSP (mm Hg) | 26.3 ± 4.9 (4) | 60.2 ± 2.1 (10)* | 56.2 ± 6.8 (5)* |
| LVSP (mm Hg) | 117.5 ± 4.3 (3) | 124.6 ± 5.7 (6) | 98.2 ± 4.7 (5)* |
| Hematocrit | 38.5 ± 1.2 (14) | 63.3 ± 2.3 (8)* | 41 ± 1 (7) |
| Fulton's Indexa | 0.28 ± 0.01 (17) | 0.57 ± 0.02 (12)* | 0.51 ± 0.03 (8)* |
| Pulmonary Artery | |||
| PHE | |||
| Max (10−5 M) contraction (g/mg) | 1.03 ± 0.11 (16) | 0.28 ± 0.05 (12)* | 0.56 ± 0.15 (12)* |
| + l-NAME (3 × 10−4 M) | 1.15 ± 0.15 (8) | 0.36 ± 0.11 (6)* | 0.65 ± 0.26 (6) |
| +ODQ (10−5 M) | 1.45 ± 0.27 (8) | 0.38 ± 0.11 (6)* | 0.81 ± 0.24 (6) |
| pED50 (−log M) | 7.64 ± 0.13 (16) | 7.57 ± 0.08 (12) | 7.43 ± 0.07 (12) |
| + l-NAME (3 × 10−4 M) | 7.93 ± 0.07 (8) | 7.71 ± 0.24 (6) | 7.53 ± 0.15 (6)* |
| +ODQ (10−5 M) | 7.86 ± 0.11 (8) | 7.94 ± 0.17 (6)** | 8.07 ± 0.06 (6)** |
| ACh (10−5 M) % relaxation | 51.2 ± 2.9 (16) | 19.5 ± 5.4% (12)* | 9.3 ± 2.8% (12)* |
| SNP (10−5 M) % relaxation | 95.2 ± 2.1 (8) | 78.2 ± 7.7 (6)* | 50.4 ± 5.5 (8)* |
| Mesenteric artery | |||
| PHE | |||
| Max (10−5 M) contraction (g) | 0.89 ± 0.09 (8) | 0.70 ± 0.12 (6) | 0.76 ± 0.11 (6) |
| pED50 (−log M) | 6.29 ± 0.15 (8) | 6.36 ± 0.16 (6) | 6.42 ± 0.20 (6) |
| ACh (10−5 M) % relaxation | 100.0 ± 0.0 (8) | 97.3 ± 1.8 (6) | 97.9 ± 1.1 (6) |
| SNP (10−5 M) % relaxation | 100.0 ± 0.0 (3) | 100.0 ± 0.0 (3) | 100.0 ± 0.0 (4) |
Data represent means ± S.E.M. (n).
a
Fulton's Index = right/(left + septum) ventricle weight.
*
, measurements in hypoxia or MCT-treated rats are significantly different (P < 0.05) from corresponding measurements in normoxic rats.
**
, measurements in L-NAME- or ODQ-treated arteries are significantly different from corresponding measurement in nontreated arteries.