FIG. 5.
(A) Bootstrap consensus tree for a neighbor-joining algorithm showing relative genetic relatedness between eight IncA/C plasmids. The tree was based on sequences from 91 conserved genes (BLAST score threshold = 0.3) (see Table S1 in the supplemental material; see Materials and Methods for details), with pIP1202 arbitrarily chosen as the reference plasmid. (B) Subtree for blaCMY-2 plasmids based on 25 conserved genes (BLAST score threshold = 0.3) (see Table S2 in the supplemental material; see Materials and Methods for details), with pSN254 used as the reference sequence. (C) Subtree for three additional plasmids based on 49 conserved genes (BLAST score threshold = 0.3; see Table S3 in the supplemental material; see Materials and Methods for details), with pIP1202 used as the reference sequence. Neighbor-joining consensus trees were calculated from 500 bootstrap iterations, with numbers at nodes representing percent correspondence. The scale bars represent the number of base substitutions per site (32).