TABLE 1.
Basic characteristics of three sequenced blaCMY-2plasmids (this study) and the reference sequence, pSN254
| Characteristic | pAR060302 (FJ621588) | peH4H (FJ621586) | pAM04528 (FJ621587) | pSN254a (NC_009140) |
|---|---|---|---|---|
| Mammalian host | Cow | Cow | Human | NA |
| Bacterial host | E. coli | E. coli | S. Newport | S. Newport |
| Strain | AR060302 | H4H | AM04528 | SN254 |
| Year of isolation | 2002 | 2002 | 1998 | NA |
| Antibiotic resistance phenotypeb | AM-AMC-C-CAZ-GM-S-SUL-TE | AM-AMC-C-CAZ-GM-K-S-SUL-TE | AM-AMC-C-CAZ- S- SUL-TE | AM-AMC-C-CAZ-GM-S-SUL-TEc |
| Size (bp) | 166,531 | 148,106 | 158,195 | 176,473 |
| %GC | 53.1 | 48.3 | 51.9 | 52.8 |
| Conjugation efficiencyd | 1.3 × 10−3 | 0.0 | 0.0 | NA |
| Giles typee | A | C | C | Cf |
| No. of copies of blaCMY-2 | 1 | 2 | 2 | 2 |
| floR regiong | Yes | Yes | Yes | Yes |
| aadA regiong | Yes | Yes | No | Yes |
| kan regiong | No | Yes | No | No |
| merA regiong | Yes | Yes | Yes | Yes |
The pSN254 sequence was published by Welch et al. (33). NA, not applicable.
AM, ampicillin; AMC, amoxicillin-clavulanic acid; C, chloremphenicol; CAZ, ceftazidime; GM, gentamicin; K, kanamycin; S, streptomycin; SUL, sulfasoxazole; TE, tetracycline.
The antibiotic resistance phenotype is inferred from reference 33 and the plasmid sequence.
Conguation efficiencies for plasmids that were first transferred to E. coli DH10B by electroporation.
Giles type based on plasmid RFLP after PstI digestion (13).
The Giles type for pSN254 is presumed to be group C based on sequence similarity with pAM04528.
The floR, aadA, kan, and merA regions are shown in Fig. 1.