FIG. 1.

(A) Schematic of the plasmid and the outcome of integration into P. falciparum D10 parasites. Plasmid pPyMEGF was constructed by ligating a chimeric MSP1 molecule (the PfMSP1 gene target with its C-terminal 19-kDa fragment [gray box] replaced with PyMSP1₁₉ [black box]) into transfection vector pHC2. The Tg-DHFR selectable marker cassette and Hsp86 3′ UTR are shown. (B) Outcome of amplification using different sets of primers on the following samples: no DNA control (lanes 1), D10 genomic DNA (lanes 2), plasmid pPyMEGF-1 (forward insert orientation) (lanes 3), plasmid pPyMEGF-2 (reverse insert orientation) (lanes 4), genomic DNA from PyMEGF-1 (lanes 5), PyMEGF-2 (lanes 6) after the second round of drug cycling. DNA standards (in bp) are shown on the left. The locations of the primers are shown in panel A.