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Comparison of the growth rates of D10-PyMEGF (triangles), D10-PfM3′ (circles), and D10 (diamonds). The medium containing pyrimethamine was changed every day, and the culture was diluted 1:5 with fresh medium and additional red blood cells at a 2% hematocrit every 32 h (indicated by arrows). Parasitemias were counted every 8 h from the duplicated wells of each parasite line. The error bars indicate standard deviations of the means.
