TABLE 1.
Strains and plasmids used in this study
| Strain | Plasmid | Description | Source or reference |
|---|---|---|---|
| P. gingivalis strains | |||
| W83 | Parent strain | 11 | |
| V2897 | Emr; FeoB1-deficient mutant | This study | |
| E. coli strains | |||
| One Shot | Chemically competent cells | Invitrogen | |
| Top 10 | pCR 2.1 | Cloning vector | Invitrogen |
| V2198 | pVA2198 | Carries 2-kb ermF-ermB cassette | 6 |
| V2762 | pVA2762 | Apr; pCR 2.1 containing the 2.2-kb feoB fragment PCR amplified from P. gingivalis W83 genomic DNA | This study |
| V2760 | pVA2760 | Apr Emr; pVA2762 with the ermF-ermB cassette inserted at the SmaI site | This study |