TABLE 2.
Primer | Position in feoB1 (bp) | Sequence (5′-3′) |
---|---|---|
Primers for feoB amplification | ||
FeoF | GATATCGGATCCATGATGAAAAAGAGTGCTACa | |
FeoR | GCAAGCGAGCTCTCCTCCGACCAACCGCCATACb | |
Primers for mutant verification | ||
FeoBF | 215-235 | CCGAAGGTGCATTCTCGTATG |
FeoBR | 632-612 | CAGTACCGATACTTGTCACTC |
Primers for qRT-PCR | ||
FeoB1-F | GAAAGCATTATCACGGATCAG | |
FeoB1-R | TGTAGCTGCTCCGGTATGTTTCT |
The BamHI site is underlined.
The XhoI site is underlined.