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Primers used in this study
| Primer | Position in feoB1 (bp) | Sequence (5′-3′) |
|---|---|---|
| Primers for feoB amplification | ||
| FeoF | GATATCGGATCCATGATGAAAAAGAGTGCTACa | |
| FeoR | GCAAGCGAGCTCTCCTCCGACCAACCGCCATACb | |
| Primers for mutant verification | ||
| FeoBF | 215-235 | CCGAAGGTGCATTCTCGTATG |
| FeoBR | 632-612 | CAGTACCGATACTTGTCACTC |
| Primers for qRT-PCR | ||
| FeoB1-F | GAAAGCATTATCACGGATCAG | |
| FeoB1-R | TGTAGCTGCTCCGGTATGTTTCT |
The BamHI site is underlined.
The XhoI site is underlined.
