Cytokine responses of P. gingivalis FK-W50-primed T cells from PAR-2+/+ and PAR-2−/− mice. T cells isolated from inguinal and popliteal lymph nodes of mice 7 days after immunization (a) and T cells isolated from submandibular lymph nodes (b) from the mouse periodontitis model were stimulated with P. gingivalis FK-W50 (1 μg/ml) in the presence of gamma-irradiated syngeneic antigen-presenting cells in vitro. After 2 days, the assay was stopped and developed. The data are expressed as SFC/million ± the SD minus the background and are the averages of triplicate assays. S-Im, sham-immunized mice; Im, immunized mice; S-Inf, sham-infected mice; Inf, infected mice.