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. 2009 Dec 7;78(2):672–679. doi: 10.1128/IAI.00808-09

FIG. 4.

FIG. 4.

Knockdown of TLR2 reversed the suppressive effect of T. denticola on HBD-3 expression. HOK-16B cells were transfected with control or TLR2-specific siRNA. After a 24-h incubation, the cells were infected with live T. denticola at an MOI of 1,000 for 24 h. (A and B) Gene silencing was assessed by real-time RT-PCR (A) and flow cytometry (B) 24 h after transfection. (C) The levels of HBD-3 transcripts were evaluated by real-time RT-PCR. The means ± the SEM of results from nine real-time RT-PCR assays are given as the percentages of expression in T. denticola-infected cells compared to expression in the control culture without bacteria. ***, P < 0.001 versus results for scramble RNAi.