FIG. 2.

The partial activation of DCs by rFhCL1 and rFhGST-si is TLR4 dependent. DCs from C57BL/6 (background strain; WT), TLR4-deficient (TLR4KO), C3H/HeN, and C3H/HeJ mice were cultured with medium (Med), rFhCL1 (10 μg/ml), rFhGST-si (10 μg/ml), zymosan (5 μg/ml), or LPS (100 ng/ml) for 24 h, and the quantities of IL-12p40 (A and D) and IL-6 (B and E) in supernatants were determined by ELISA. Data are presented as means (± SEM) and are representative of three experiments. ***, P ≤ 0.001 compared to control mice. (C and F) Dendritic cells were harvested and analyzed by four-color flow cytometry, gated on CD11c⁺ cells, for the expression of CD40. In each histogram, the stimulated cells are represented by the black line and the isotype control by the filled histogram. Numbers represent mean fluorescence intensities (MFI) for three separate experiments.