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rFhGST-si and rFhCL1 suppress the ability of DCs to secrete IL-23 in response to LPS. DCs from C57BL/6 mice were cultured with medium, rFhCL1, or rFhGST-si (10 μg/ml) 2.5 h prior to LPS stimulation (100 ng/ml). After 18 h, IL-23 in the supernatants was measured by sandwich ELISA. Data are presented as means (± SEM) and are representative of three experiments. ***, P ≤ 0.001 compared with LPS group.
