FIG. 3.

Effect of MβCD on Ca²⁺ influx facilitated by TDH. (A) Ca²⁺ influx into HeLa cells facilitated by TDH or R7. HeLa cells were pretreated with 4 mM MβCD or not for 1 h, followed by Fluo 4-AM loading for 30 min. Cells were incubated with TDH or R7 for 30 min, and intracellular Ca²⁺ levels of cells were determined. Black squares, TDH treated; white triangles, MβCD pretreated and TDH treated; white squares, R7 treated. (B) Cytotoxicity of TDH or R7 for HeLa cells for 30 min. Black squares indicate that HeLa cells were treated with TDH, white triangles indicate that HeLa cells were pretreated with 4 mM MβCD for 1 h and treated with TDH, and white squares indicate that HeLa cells were treated with R7. Data are from three experiments ± SD.