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Depletion of sphingomyelin affects association of TDH with lipid rafts. HeLa cells were incubated with 100 mU/ml SMase or no SMase for 1 h and then exposed to 10 μg/ml TDH for 30 min. DRMs were prepared and analyzed by immunoblotting against TDH, Cav-1, Flt-1, and TfR. (A) Nontreated; (B) SMase treated.
