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Distribution of TDH on cells by fluorescent microscopy. HeLa cells were incubated with TDH-Myc in the presence of Alexa 546-CTxB (A) or Alexa 546-Tfn (B) and fixed. The cells were then probed with anti-c-Myc antibody and subsequently stained with Alexa 488-conjugated anti-mouse antibody. Images were obtained by fluorescent microscopy. Arrowheads indicate nonspecific signals that were observed with anti-c-Myc antibody even in the absence of TDH-Myc (data not shown).
