TABLE 2.
Primers used in the study
| Primer | Sequence (5′-3′)a |
|---|---|
| NCHKs | CGACCTGTCCTGGTGATGC |
| Cnf1-s3 | GCGCGGTACCATGGGTAACCAATGGCAA |
| Cnf1-a | GGATCCGGTACCAAATTTTTTTGAAATACCTTCA |
| NC-s3 | GGCGTCTAGATTTTGATTCGGGAAATTATT |
| NC-a3 | GGCGCCATGGACTCTGCCCGATGATTTTC |
| NN-s | GTTGAAGTACTGGCTGTGGTT |
| NFB-CKS | GCTACTGAGGAAGAAGCATGGAA |
| NFB-CKA | TCGCAGGTGAGCCGAAACT |
| GRGM-f | TTTCACTTATCTGGTTGGTCG |
| GRGM-r | CGAGGCTTGTCAGTACATCA |
| AraCP-s | CCGGCCTAGGCTGATTCGTTACCAATTATGAC |
| AraCP-a | CCGGCTCGAGCATGGTTAATTCCTCCTGTTA |
| fdx-KOF | TTCGCCAATTTCGCGGCTATCCGTCCACTTAAGTCCCATACTAACCTCTGGTGTAGGCTGGAGCTGCTTC |
| fdx-KOR | CCAGTCGGTTCGTCGTGCGCTGAAAGGCCATTCCGTGGACGAGGTTTAATCATATGAATATCCTCCTTAG |
| FdxCKF | AGGATTTTCTCGTTGGATG |
| FdxCKR | AAGACTCAATGAGCTATGCC |
| fdx-a | CGCGCGGCCGCACTTAAGTCCCATACTAACCTC |
| fdx-s | GCGCGCATATGCCAAAGATTGTTATTTTG |
| Spc-SeqR | GCCTTGCTGTTCTTCTACGG |
| Tn7-ckf | ACGGTCGGGAACTGGAAC |
| Tn7-ckr | TGACCAGCCGCGTAACCT |
a
Restriction sites for cloning are underlined.