TABLE 1.
Spot | Proteinc | AVRa |
gi no. | pI | Molecular mass (kDa) | Functionb | Reference(s) | ||
---|---|---|---|---|---|---|---|---|---|
H/C | M/C | H/M | |||||||
1 | Actin | +2.12 | +1.81 | +1.17 | 809561 | 5.8 | 39.45 | STR | 25 |
2 | Actin | +2.39 | +2.04 | +1.17 | 49868 | 5.8 | 39.45 | STR | 25 |
3 | Albumin | +3.26 | +1.88 | +1.73 | 19353306 | 5.8 | 70.73 | APP | 25 |
4 | Albumin | +2.84 | +1.71 | +1.66 | 33859506 | 5.8 | 70.73 | APP | 25 |
5 | Albumin | +2.83 | +1.73 | +1.63 | 19353306 | 5.8 | 70.73 | APP | 25 |
6 | Albumin | −2.13 | −1.51 | −1.41 | 26341396 | 5.5 | 67.04 | APP | 25 |
7 | Aldehyde dehydrogenase 9 | +2.87 | +1.90 | +1.51 | 9910128 | 6.6 | 54.47 | ROX | 17, 64 |
8 | Annexin 5 | +4.52 | +2.17 | +2.08 | 6753060 | 4.8 | 35.78 | SIG | 43 |
9 | ATPS | −2.05 | −1.88 | −1.09 | 23272966 | 5.2 | 56.65 | EGM | 11, 25 |
10 | ATPS | −2.13 | −1.72 | −1.24 | 23272966 | 5.2 | 56.65 | EGM | 11, 25 |
11 | Betaine homocysteine methyltransferase 2 | +3.80 | +2.54 | +1.50 | 62533211 | 6.0 | 40.28 | MYL | 20, 59 |
12 | Brain and reproductive organ-expressed protein | +3.04 | +2.08 | +1.46 | 38173925 | 5.7 | 43.95 | APO | 12, 36 |
13 | Carbamoyl phosphate synthase | −2.60 | −1.87 | −1.39 | 73918911 | 6.5 | 165.8 | EGM | 25, 60 |
14 | Chaperonin-containing TCP-1 theta subunit | −2.85 | −1.78 | −1.60 | 5295992 | 5.4 | 60.06 | PRF | 1, 25 |
15 | Collagen 6a1 | +17.8 | +12.2 | +1.45 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
16 | Collagen 6a1 | +47.8 | +32.3 | +1.48 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
17 | Collagen 6a1 | +55.8 | +36.2 | +1.54 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
18 | Collagen 6a1 | +47.8 | +31.9 | +1.50 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
19 | Collagen 6a1 | +34.7 | +24.2 | +1.43 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
20 | Collagen 6a1 | +21.0 | +15.5 | +1.36 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
21 | Collagen 6a1 | +10.2 | +7.81 | +1.30 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
22 | Collagen 6a1 | +5.38 | +4.94 | +1.09 | 12805443 | 5.8 | 44.76 | STR | 19, 25 |
23 | Collagen XIV | +5.13 | +2.40 | +2.13 | 30420885 | 5.0 | 194.3 | STR | 19, 25 |
24 | Collagen XIV | +4.27 | +2.78 | +1.54 | 30420885 | 5.0 | 194.3 | STR | 19, 25 |
25 | Collagen XIV | +7.13 | +3.61 | +1.97 | 30420885 | 5.0 | 194.3 | STR | 19, 25 |
26 | DMDH | −2.04 | −1.71 | −1.20 | 59808083 | 7.8 | 97.44 | CHO | 25 |
27 | DMDH | −2.21 | −1.74 | −1.27 | 59808083 | 7.8 | 97.44 | CHO | 25 |
28 | DMDH | −2.34 | −1.76 | −1.33 | 59808083 | 7.8 | 97.44 | CHO | 25 |
29 | EH | −2.46 | −1.78 | −1.38 | 15929294 | 5.9 | 63.07 | XBM | 44 |
30 | EH | −2.41 | −2.03 | −1.19 | 15929294 | 5.9 | 63.07 | XBM | 44 |
31 | EH | −2.82 | −1.85 | −1.52 | 15929294 | 5.9 | 63.10 | XBM | 44 |
32 | 10-FTHFDH | −2.52 | −1.49 | −1.69 | 20380027 | 5.6 | 99.55 | MYL | 33, 64 |
33 | 10-FTHFDH | −2.56 | −1.43 | −1.79 | 20380027 | 5.6 | 99.55 | MYL | 33, 64 |
34 | 10-FTHFDH | −2.28 | −1.54 | −1.48 | 23271467 | 5.6 | 99.55 | MYL | 33, 64 |
35 | 10-FTHFDH | −2.36 | −1.51 | −1.56 | 20380027 | 5.6 | 99.55 | MYL | 33, 64 |
36 | 10-FTHFDH | −2.47 | −1.70 | −1.46 | 23271467 | 5.6 | 99.55 | MYL | 33, 64 |
37 | Glucosidase II β | +3.53 | +2.47 | +1.43 | 57013837 | 4.4 | 59.74 | PRF | 2, 49 |
38 | Glucosidase II β | +2.47 | +2.13 | +1.16 | 57013837 | 4.4 | 59.74 | PRF | 2, 49 |
39 | Group-specific component | +2.89 | +1.81 | +1.60 | 51172612 | 5.4 | 55.18 | APP | 40, 55 |
40 | Keratin D | +2.43 | +1.52 | +1.60 | 293682 | 5.3 | 47.47 | STR | 9, 53 |
41 | Lymphocyte cytosolic protein 1 (plastin 2) | +4.17 | +3.06 | +1.36 | 26326929 | 5.2 | 70.77 | PRF | 57 |
42 | Plastin 2 | +3.24 | +2.45 | +1.32 | 26326929 | 5.2 | 70.77 | PRF | 57 |
43 | MUP | −8.11 | −1.69 | −4.80 | 53271 | 4.8 | 17.71 | PHE | 18 |
44 | MUP | −7.54 | −1.50 | −5.02 | 12851568 | 4.8 | 17.71 | PHE | 18 |
45 | MUP | −10.3 | −2.61 | −3.96 | 13276755 | 4.8 | 17.71 | PHE | 18 |
46 | MUP | −5.56 | −2.35 | −2.37 | 494384 | 4.8 | 17.71 | PHE | 18 |
47 | MUP | −3.51 | −1.50 | −2.34 | 38488789 | 4.8 | 21.73 | PHE | 18 |
48 | Myosin light polypeptide 6 | −2.15 | −1.68 | −1.28 | 33620739 | 4.6 | 17.09 | STR | 66 |
49 | NADH dehydrogenase (ubiquinone) Fe-S protein 1 | −2.12 | −1.75 | −1.21 | 21704020 | 5.5 | 80.76 | EGM | 8 |
50 | Protein disulfide-isomerase A3, Erp57 | +3.17 | +1.94 | +1.63 | 26353794 | 5.8 | 57.12 | ROX | 25, 54 |
51 | Pyruvate carboxylase | −3.44 | −2.17 | −1.58 | 26346581 | 6.1 | 103.8 | EGM | 24, 25 |
52 | Pyruvate carboxylase | −2.35 | −1.72 | −1.36 | 26346581 | 6.1 | 103.8 | EGM | 24, 25 |
53 | Retinol binding protein type I | +2.73 | +1.91 | +1.43 | 21730472 | 5.1 | 15.86 | APP | 61 |
54 | RNase/angiogenesis inhibitor | +2.15 | +2.16 | −1.00 | 14577933 | 4.6 | 51.21 | IMM | 14 |
55 | S-Adenosylhomocysteine hydrolase | −2.10 | −1.68 | −1.25 | 63471580 | 6.1 | 48.08 | MYL | 35 |
56 | SARDH | −2.16 | −1.35 | −1.59 | 26352359 | 6.4 | 57.84 | CHO | 37 |
57 | SARDH | −2.80 | −1.46 | −1.92 | 26352359 | 6.4 | 57.84 | CHO | 37 |
58 | SARDH | −6.43 | −3.23 | −1.99 | 26352359 | 6.4 | 57.84 | CHO | 37 |
59 | SBP2 | −3.66 | −2.40 | −1.53 | 188848341 | 5.8 | 53.16 | XBM | 25, 28 |
60 | SBP2 | +2.99 | +1.63 | +1.84 | 18848341 | 6.0 | 52.90 | XBM | 25, 28 |
61 | Succinate dehydrogenase Fp subunit | −2.33 | −1.81 | −1.29 | 15030102 | 6.2 | 59.27 | EGM | 8 |
62 | Transferrin | +2.83 | +1.43 | +1.98 | 62027488 | 7.0 | 78.87 | APP | 25 |
63 | Transferrin | +4.17 | +1.66 | +2.52 | 62027488 | 7.0 | 78.87 | APP | 25 |
64 | Transferrin | +4.72 | +1.71 | +2.76 | 62027488 | 7.0 | 78.87 | APP | 25 |
65 | Transferrin | +4.60 | +1.68 | +2.74 | 62027488 | 7.0 | 78.87 | APP | 25 |
66 | Tropomyosin 3 | +2.19 | +1.61 | +1.37 | 26341416 | 4.7 | 27.88 | STR | 25, 46 |
67 | Valosin-containing protein | +4.76 | +3.68 | +1.29 | 26350783 | 5.1 | 80.70 | PRF | 68 |
68 | Vimentin | +2.31 | +2.67 | −1.16 | 31982755 | 4.9 | 53.73 | STR | 9, 25 |
69 | Vimentin | +2.54 | +2.68 | −1.06 | 31982755 | 4.9 | 53.73 | STR | 9, 25 |
70 | Aldehyde dehydrogenase A1 | −2.62 | −2.02 | −1.41 | 32484332 | 8.3 | 55.08 | ROX | 17, 64 |
71 | Catalase 1 | −3.00 | −2.61 | −1.15 | 15004258 | 7.8 | 60.01 | ROX | 25, 48 |
72 | Glutamate dehydrogenase | −2.76 | −2.12 | −1.30 | 26354278 | 8.6 | 61.60 | EGM | 42, 63 |
73 | Glutathione S-transferase pi class | −3.27 | −2.05 | −1.59 | 2624496 | 8.3 | 23.52 | XBM | 25, 26 |
74 | H2 Q4 | +5.01 | +3.23 | −1.55 | 51770518 | 7.3 | 71.74 | IMM | 22, 29 |
75 | H2 Q4 | +8.09 | +4.88 | −1.66 | 51770518 | 7.3 | 71.74 | IMM | 22, 29 |
76 | H2 Q4 | +12.2 | +7.29 | −1.67 | 51770518 | 7.3 | 71.74 | IMM | 22, 29 |
77 | H2 Q4 | +18.3 | +11.6 | −1.58 | 51770518 | 7.3 | 71.74 | IMM | 22, 29 |
78 | H2 Q4 | +17.2 | +10.4 | −1.66 | 51770518 | 7.3 | 71.74 | IMM | 22, 29 |
79 | sm-Phosphoenolpyruvate carboxykinase | +14.4 | +8.05 | −1.79 | 74828716 | 6.8 | 71.46 | GLN | 5 |
80 | Prohibitin 2 | +3.25 | +1.61 | +2.02 | 61556754 (76363295) | 9.8 | 33.29 | PRF | 6, 41 |
AVR: average volume ratio between study groups (C, control; M, MSS; H, HSS).
APO, antiapoptotic; APP, acute-phase protein; CHO, choline metabolism; EGM, energy metabolism; GLN, gluconeogenesis; IMM, immune response; MYL, methylation; PHE, pheromone; PRF, protein folding; PRM, protein metabolism; ROX, redox reactions; SIG, cell signaling; STR, structural protein; XBM, xenobiotic metabolism.
ATPS, ATP5 beta synthetase; DMDH, dimethylglycine dehydrogenase; EH, epoxide hydrolase; 10-FTHFDH, formyl tetrahydrofolate dehydrogenase; SARDH, sarcosine dehydrogenase; H2 Q4, MHC class I H2 Q4 alpha chain.