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. 2009 Nov 16;78(2):810–822. doi: 10.1128/IAI.00926-09

FIG. 4.

FIG. 4.

rTcPRAC-specific IgG is undetectable during experimental T. cruzi infection. Susceptible BALB/c or resistant C57BL/6 mice were infected with T. cruzi. (A) Serial serum samples were analyzed for responses to whole parasites, rTcPRAC, or negative control to establish background binding. Data represent triplicate repeats with pooled sera from five mice in each experiment. (B) Western blot analysis of IgG reactivity to rCRP (500 ng) (lane 1), rTcPRAC (500 ng) (lane 2), and parasite membrane proteins (10 μg) (lane 3) in BALB/c sera collected at 125 days postinfection (diluted 1:500).