FIG. 1.

Ephrin B1 deficiency in osteoblasts caused skull defects and reduced body size. (A) Breeding scheme for generation of ephrin B1 (efnb1) conditional KO and WT mice. (B) Loss of ephrin B1 expression in osteoblasts from ephrin B1 conditional KO mice measured by Western blotting. (C) Female embryos at E19.5. The arrow indicates the exencephaly. (D) Alizarin red and alcian blue staining of the skulls of the same embryos as in panel C. Arrows indicate skull defects. (E) Alizarin red and alcian blue staining of the same embryo skeletons as in panel C. Arrows indicate calvarial defects. (F) Body sizes of 3-week-old WT and KO mice. (G) Body lengths of WT and KO mice at 3 and 8 weeks of age (n = 14).