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. 2009 Nov 23;30(3):806–819. doi: 10.1128/MCB.00569-09

FIG. 7.

FIG. 7.

Pin1 contributes to the recycling of oncogenic B-Raf proteins. (A) NIH 3T3 cells expressing V600E, G466A, or D594G B-Raf protein were treated or not with 0.5 μM OA for 1 h. Lysates were prepared, and the electrophoretic mobility of the B-Raf proteins was examined by immunoblot analysis. (B) The indicated Flag-B-Raf proteins were immunoprecipitated (IP) and examined for binding of endogenous (End.) Pin1 by immunoblot analysis. The blot was reprobed to confirm equivalent Flag-B-Raf levels. (C) Lysates from control NIH 3T3 cells or cells stably expressing either WT or DN Pin1 were compared for Pin1 expression levels. (D) Control NIH 3T3 cells or cells stably expressing either WT or DN Pin1 were infected with recombinant retroviruses expressing the indicated B-Raf proteins. Focus formation was visualized after 2 weeks in culture. Focus plates from a representative experiment are shown. The mean number of foci ± standard deviation from 3 independent experiments is given below each plate.