Skip to main content
. 2009 Nov 23;30(3):578–589. doi: 10.1128/MCB.01137-09

FIG. 2.

FIG. 2.

Southern blot analysis for identification of B3-Zeo clones in which the pZTIL-tel plasmid has been targeted to replace the pNCT-tel plasmid in clone B3. (a) The structure of the integrated plasmid sequences and the sizes of the predicted HindII restriction fragments are shown for the parental clone B3 and for clones containing the pZTIL-tel plasmid (B3-Zeo). (b) Southern blot analysis using the pNCTΔ plasmid of genomic DNA from clone B3 results in three HindIII restriction fragments (4.2, 2.3, and 2.0 kb) (arrows at left). B3-Zeo clones containing the targeted pZTIL-tel plasmid (clones 37, 59, and 95) contain two HindIII restriction fragments (4.2 and 1.8 kb; arrows at right). The location of the HindIII (H) restriction sites, the recognition site for the I-SceI endonuclease, and the recognition site for Cre recombinase (loxP) are shown. The molecular size markers (kb) consist of lambda bacteriophage HindIII fragments. This figure was generated by combining data from different Southern blots containing genomic DNA from B3-Zeo clones.