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. 2009 Nov 23;30(3):578–589. doi: 10.1128/MCB.01137-09

FIG. 3.

FIG. 3.

The structure of the integrated plasmid sequences in the various cell clones used in this study. Clone B3-Zeo37 contains a single copy of the pZTIL-tel plasmid located adjacent to the telomere on the short arm of chromosome 16. Clone 10-7 was derived by targeted integration of the pNTIL-100kb plasmid to a site 100 kb from the telomeric pZTIL-tel plasmid in clone B3-Zeo37. The targeted integration of pNTIL-100kb resulted in the duplication of the target sequence on either side of the integrated plasmid, typical of insertion-type targeting vectors (56). The 10-7Cre clones were derived from 10-7 following Cre recombinase-mediated recombination between the LoxP sites, which resulted in the repositioning of the telomere to a location immediately adjacent to the pNTIL-100kb plasmid. The location of restriction sites and the size of restriction fragments are shown for the BglII (B), HindIII (H), NcoI (N), and XbaI (X) restriction enzymes. Also shown are the locations of the I-SceI and LoxP sites (boldface I and L, respectively) recognized by the I-SceI endonuclease and Cre recombinase, respectively. Symbols are shown for the location of the telomere, plasmid vector sequences (Amp/ori), Neo gene, HSV-tk gene, Zeo gene, and cellular sequence used for targeted integration of pNTIL-100kb (Target site).