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. 2009 Nov 23;30(3):578–589. doi: 10.1128/MCB.01137-09

FIG. 5.

FIG. 5.

Decreased expression of transgenes following the relocation of the telomere to a site adjacent to the pNTIL-100kb plasmid. q-RT-PCR was used to determine the level of expression of the Neo gene and the HSV-tk gene in the parental clone 10-7 and four clones (10-7Cre1, 10-7Cre2, 10-7Cre3, and 10-7Cre6), that were derived from 10-7 following Cre-mediated recombination between LoxP sites in the pNTIL-100kb and pZTIL-tel plasmids. The results are given as percentage of the level of GAPDH gene expression. Error bars represent the standard deviation from three separate experiments. The levels of expression of both the Neo and HSV-tk genes were significantly reduced in all four Cre clones compared to clone 10-7 (P < 0.0001 for Neo and HSV-tk in Cre1, Cre 3, and Cre6; P < 0.0004 and P < 0.001 for Neo and HSV-tk, respectively, in Cre2).