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. 2009 Nov 30;30(3):613–625. doi: 10.1128/MCB.00685-09

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FIG. 4. — ATF-2 is required for maximal PPARγ2 transcription. (A) Adipocyte differentiation assay. MEFs from WT, Atf-2^+/⁻ Cre-bpa^+/⁻, or Atf-2⁻^/⁻ Cre-bpa⁻^/⁻ mice were induced to differentiate into adipocytes using differentiation medium containing BMP-2. Oil-Red O staining shows fat accumulation in cells at 8 days after differentiation induction. Bar, 5 mm. (B) Reduced levels of PPARγ2 mRNA in Atf-2⁻^/⁻ Cre-bpa⁻^/⁻ MEFs. The levels of PPARγ2 mRNA during adipocyte differentiation of WT and Atf-2⁻^/⁻ Cre-bpa⁻^/⁻ MEFs were measured by real-time RT-PCR. The levels (mean ± SD; n = 3) are shown relative to preinduction levels of WT MEFs. (C) Reduced levels of PPARγ2 mRNA in adipose tissues of Atf-2^+/⁻Cre-bpa^+/⁻ mice. The levels of PPARγ2, C/EBPα, C/EBPβ, and C/EBPδ mRNAs in adipose tissues of Atf-2^+/⁻ Cre-bpa^+/⁻ and WT mice were measured by real-time RT-PCR and are shown relative to WT levels (mean ± SD; n = 3). *, P < 0.05; N.S., no significant difference. (D) Key transcription factor mRNA levels. The levels of C/EBPα, C/EBPβ, C/EBPδ, and Id mRNAs during adipocyte differentiation of WT and Atf-2⁻^/⁻ Cre-bpa⁻^/⁻ MEFs were measured by real-time RT-PCR. The levels (mean ± SD; n = 3) are shown relative to preinduction levels of WT MEFs.