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. 2009 Nov 30;30(3):613–625. doi: 10.1128/MCB.00685-09

FIG. 6.

FIG. 6.

Adipocyte differentiation is positively regulated by p38 via PPARγ2 expression. (A) Rescue of adipocyte differentiation of Atf-2−/− Cre-bpa−/− MEFs by overexpression of PPARγ2. Adipocyte differentiation of Atf-2−/− Cre-bpa−/− and WT MEFs was induced using differentiation medium containing BMP-2. PPARγ2 was expressed using a viral expression vector, while control cells were infected with virus harboring the empty vector. Bar, 2 μm. (B) Inhibition of adipocyte differentiation by p38 inhibitors. WT MEFs were induced to differentiate into adipocytes using differentiation medium with (frames iv and v) or without (frames i to iii) BMP-2. In some cases, p38 inhibitor FR167653 or SB203580 was added. Oil-Red O staining shows fat accumulation in cells at 8 days after induction of differentiation. Bar, 5 mm. (C) The effect of the p38 inhibitor FR167653 on adipocyte differentiation is blocked by overexpression of PPARγ2. Adipocyte differentiation of WT MEFs was induced using differentiation medium without BMP, as described above. In some cases, the p38 inhibitor FR167653 was added, or PPARγ2 or C/EBPα was expressed using a viral expression vector. Control cells were infected with virus harboring the empty vector. Bar, 5 mm.