TABLE 1.
Performance of C Diff Quik Chek EIA, Tox A/B Quik Chek EIA, CYT assay, and direct PCR for detection of toxigenic C. difficile
| No. of samples | Result by assaya: |
Interpretation of results | |||
|---|---|---|---|---|---|
| GDH-Q | A/B-Q | CYT | DPCRb | ||
| 585 | − | ND | − | ND | − |
| 43 | + | − | − | − | − |
| 2 | + | + | − | ND | − |
| 26 | + | + | + | ND | + |
| 19 | + | − | + | + | + |
| 2 | + | − | + | − | + |
| 22 | + | − | − | + | + |
a
Abbreviations: GDH-Q, C Diff Quik Chek EIA; AB-Q, Tox A/B Quik Chek EIA; CYT, cytotoxin tissue culture assay; DPCR, direct real-time PCR detection of tcdB; ND, not done.
b
PCR results: 1.9% (4/211) of consecutive GDH-Q-negative specimens in a subsequent time period were DPCR positive for tcdB; therefore, 11 of 585 are expected to be GDH-Q negative, DPCR positive, as shown in Table 2.