TABLE 1.
Primers used for multiplex PCR to detect all nine copies of ISBcen20 present in the genome of B. cenocepacia J2315
| Insertion point (gene no. of ISBcen20) | Sequence (5′ to 3′) | Product size (bp) | Primer concn (μM) | Multiplex PCR no. |
|---|---|---|---|---|
| Reverse primers | ||||
| 1 (BCAL0573) | AGGAAATCGCCGCGATTC | 322 | 1 | M1 |
| 2 (BCAL1125) | TCTCGAACTGGAAGTGCC | 387 | 0.75 | M2 |
| 3 (BCAL1133) | TCGACGAAAGGGTGACAC | 491 | 0.4 | M3 |
| 4 (BCAL2480b) | TCGAACCGGAACTTCTCC | 557 | 1 | M1 |
| 5 (BCAL2496) | CAGACTGGGTTGATCGAG | 639 | 1.5 | M2 |
| 6 (BCAL3126b) | TCTCCTTGTCGAGTCGAC | 725 | 1.25 | M3 |
| 7 (BCAL3484) | TTCGATCCTGCGTTCGAC | 821 | 1 | M1 |
| 8 (BCAM1906) | TGCAGAAGCAAGGGCATC | 896 | 1.5 | M2 |
| 9 (BCAM1925) | TGTTCCCGTATATCGGGC | 944 | 1.75 | M3 |
| Forward primer, 1 to 9 | TCAGTTGTTCCGATGCCTG | |||