TABLE 1.
Primers used to genotype pfcrt, pfmdr1, pfdhfr, and pfdhps
| Primer | Sequence (5′ → 3′) | Purpose |
|---|---|---|
| Crt1F | CATTGTCTTCCACATATATGACATAAA | Outer PCR for pfcrt |
| Crt4R | GATCTCTATACCTTCAACATTATTCCT | Outer PCR for pfcrt |
| Crt2F | TTTCCCTTGTCGACCTTAACAGATGGC | Nested PCR for the former part of pfcrt |
| Crt12R | ATCCTATTTTACCTCTACGACTGT | Nested PCR for the former part of pfcrt |
| Crt11F | TTTCTTATAGGCTATGGTATCCTTT | Nested PCR for the latter part of pfcrt |
| Crt15R | TTTTAATTTCACACTTACCAAAGT | Nested PCR for the latter part of pfcrt |
| MDR2F | AAAGATGGTAACCTCAGTATCAAAGAAGA | Outer and nested PCRs for the former part of pfmdr1 |
| MDR8R | ATGATTCGATAAATTCATCTATAGCAGCAA | Outer and nested PCRs for the former part of pfmdr1 |
| MDR10R | TTTTTTGGACACATCAACAACATCAGAATC | Nested PCR for the former part of pfmdr1 |
| MDR5F | AGAAGATTATTTCTGTAATTTGATAGAAAAAGC | Nested PCR for the former part of pfmdr1 |
| DHFR1F | ATGATGGAACAAGTCTGCGACGTTTTCGAT | PCR for pfdhfr |
| DHFR2R | TTCATTTAACATTTTATTATTCGTTTTCTT | PCR for pfdhfr |
| DHPS1F | CCATTCCTCATGTGTATACAACAC | PCR for pfdhps |
| DHPS2R | GTTTTAATCACATGTTTGCACTTTC | PCR for pfdhps |