Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Nov;61(11):4767–4771. doi: 10.1128/iai.61.11.4767-4771.1993

An 88-kilodalton antigen secreted by Aspergillus fumigatus.

H Kobayashi 1, J P Debeaupuis 1, J P Bouchara 1, J P Latge 1
PMCID: PMC281232  PMID: 8406876

Abstract

An 88-kDa component secreted in vitro by Aspergillus fumigatus has been purified by sequential chromatographic procedures. The molecule is a glycoprotein with an N-linked sugar moiety composed of mannose glucose, and galactose (16:10:1). It is recognized by antibodies from patients with aspergilloma and has potential for the immunodiagnosis of aspergilloma. The antigenicity is associated with the polypeptide part of the molecule (79 kDa).

Full text

PDF
4770

Images in this article

4769Image
on p.4769
4769Image
on p.4769
4770Image
on p.4770
4770Image
on p.4770

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernstein J. A., Zeiss C. R., Greenberger P. A., Patterson R., Marhoul J. F., Smith L. L. Immunoblot analysis of sera from patients with allergic bronchopulmonary aspergillosis: correlation with disease activity. J Allergy Clin Immunol. 1990 Oct;86(4 Pt 1):532–539. doi: 10.1016/s0091-6749(05)80209-x. [DOI] [PubMed] [Google Scholar]
  2. Burnie J. P., Matthews R. C. Heat shock protein 88 and Aspergillus infection. J Clin Microbiol. 1991 Oct;29(10):2099–2106. doi: 10.1128/jcm.29.10.2099-2106.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coleman R. M., Kaufman L. Use of the immunodiffusion test in the serodiagnosis of aspergillosis. Appl Microbiol. 1972 Feb;23(2):301–308. doi: 10.1128/am.23.2.301-308.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fratamico P. M., Buckley H. R. Identification and characterization of an immunodominant 58-kilodalton antigen of Aspergillus fumigatus recognized by sera of patients with invasive aspergillosis. Infect Immun. 1991 Jan;59(1):309–315. doi: 10.1128/iai.59.1.309-315.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gold J. W., Fisher B., Yu B., Chein N., Armstrong D. Diagnosis of invasive aspergillosis by passive hemagglutination assay of antibody. J Infect Dis. 1980 Jul;142(1):87–94. doi: 10.1093/infdis/142.1.87. [DOI] [PubMed] [Google Scholar]
  6. Gordon M. A., Lapa E. W., Kane J. Modified indirect fluorescent-antibody test for aspergillosis. J Clin Microbiol. 1977 Aug;6(2):161–165. doi: 10.1128/jcm.6.2.161-165.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hearn V. M. Antigenicity of Aspergillus species. J Med Vet Mycol. 1992;30(1):11–25. [PubMed] [Google Scholar]
  8. Hearn V. M., Griffiths B. L., Gorin P. A. Structural analysis of water-soluble fractions obtained from Aspergillus fumigatus mycelium. Glycoconj J. 1989;6(1):85–100. doi: 10.1007/BF01047892. [DOI] [PubMed] [Google Scholar]
  9. Hearn V. M., Wilson E. V., Latgé J. P., Mackenzie D. W. Immunochemical studies of Aspergillus fumigatus mycelial antigens by polyacrylamide gel electrophoresis and western blotting techniques. J Gen Microbiol. 1990 Aug;136(8):1525–1535. doi: 10.1099/00221287-136-8-1525. [DOI] [PubMed] [Google Scholar]
  10. Holmberg K., Berdischewsky M., Young L. S. Serologic immunodiagnosis of of invasive aspergillosis. J Infect Dis. 1980 May;141(5):656–664. doi: 10.1093/infdis/141.5.656. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Latgé J. P., Moutaouakil M., Debeaupuis J. P., Bouchara J. P., Haynes K., Prévost M. C. The 18-kilodalton antigen secreted by Aspergillus fumigatus. Infect Immun. 1991 Aug;59(8):2586–2594. doi: 10.1128/iai.59.8.2586-2594.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Philpot C. M., Mackenzie D. W. Detection of antibodies to Aspergillus fumigatus in ager gel with different antigens and immunodiffusion patterns. J Biol Stand. 1976 Apr;4(2):73–79. doi: 10.1016/0092-1157(76)90015-9. [DOI] [PubMed] [Google Scholar]
  14. Samuelsen H., Karlsson-Borgå A., Paulsen B. S., Wold J. K., Rolfsen W. Purification of a 20 kD allergen from Aspergillus fumigatus. Allergy. 1991 Feb;46(2):115–124. doi: 10.1111/j.1398-9995.1991.tb00554.x. [DOI] [PubMed] [Google Scholar]
  15. Schaefer J. C., Yu B., Armstrong D. An aspergillus immunodiffusion test in the early diagnosis of aspergillosis in adult leukemia patients. Am Rev Respir Dis. 1976 Mar;113(3):325–329. doi: 10.1164/arrd.1976.113.3.325. [DOI] [PubMed] [Google Scholar]
  16. Schønheyder H., Andersen P. IgG antibodies to purified Aspergillus fumigatus antigens determined by enzyme-linked immunosorbent assay. Int Arch Allergy Appl Immunol. 1984;74(3):262–269. doi: 10.1159/000233555. [DOI] [PubMed] [Google Scholar]
  17. Stynen D., Sarfati J., Goris A., Prévost M. C., Lesourd M., Kamphuis H., Darras V., Latgé J. P. Rat monoclonal antibodies against Aspergillus galactomannan. Infect Immun. 1992 Jun;60(6):2237–2245. doi: 10.1128/iai.60.6.2237-2245.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Warnock D. W. Indirect immunofluorescence test for the detection of Aspergillus fumigatus antibodies. J Clin Pathol. 1974 Nov;27(11):911–912. doi: 10.1136/jcp.27.11.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilson E. V., Hearn V. M. Use of Aspergillus fumigatus mycelial antigens in enzyme-linked immunosorbent assay and counter-immunoelectrophoresis. J Med Microbiol. 1983 Feb;16(1):97–105. doi: 10.1099/00222615-16-1-97. [DOI] [PubMed] [Google Scholar]
  20. Yu B., Niki Y., Armstrong D. Use of immunoblotting to detect Aspergillus fumigatus antigen in sera and urines of rats with experimental invasive aspergillosis. J Clin Microbiol. 1990 Jul;28(7):1575–1579. doi: 10.1128/jcm.28.7.1575-1579.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES