TABLE 1.
HIV-1 Env pseudovirus panel
| HIV-1 isolatea | Tier | Subtypeb | Country of origin | Stage of infectionc | Mode of transmissiond | Sourcee | GenBank accession no.f | ID50 titer (μg/ml)g |
|||
|---|---|---|---|---|---|---|---|---|---|---|---|
| HIVIG | 447-52D | 17b | 1.5E | ||||||||
| MW965.26 | 1A | C | Malawi | Fiebig VI | M-F | ccPBMC | U08455 | <0.02 | 0.05 | 0.15 | >50 |
| SF162.LS | 1A | B | USA | Fiebig VI | M-M | CSF | EU123924 | 6 | <0.02 | 0.8 | 2.3 |
| MN/H9 | 1A | B | USA | Fiebig VI | Mother to child | TCLA | NA | 7 | <0.02 | 0.2 | 25.4 |
| DJ263.8 | 1B | 02_AG | Djibouti | Fiebig VI | Sexual | ccPBMC | AF063223 | 89 | 20.5 | 48.3 | >50 |
| 00836-2.5 | 1B | C | India | Fiebig VI | M-F | ccPBMC | EF117265 | 455 | >50 | >50 | >50 |
| Bx08.16 | 1B | B | France | Fiebig VI | Unknown | ccPBMC | AY713411 | 81 | 0.1 | 0.9 | 10.1 |
| BaL.26 | 1B | B | USA | Fiebig VI | Mother to child | Lung | DQ318211 | 69 | 0.04 | 11.4 | 15.6 |
| HXB2.DG | 1B | B | France | AIDS | M-M | TCLA | K03455 | 28 | <0.02 | 1.0 | 1.0 |
| 271-11 | 1B | 02_AG | Cameroon | Acute/early | Unknown | ucPBMC | EU513197 | 344 | >50 | 18.6 | >50 |
| 242-14 | 1B | 02A1 | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513188 | 583 | >50 | >50 | >50 |
| 25710-2.43 | 1B | C | India | Fiebig V | F-M | ccPBMC | EF117271 | 311 | >50 | >50 | >50 |
| H077.31 | 1B | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210734 | 783 | >50 | >50 | >50 |
| 6535.3 | 1B | B (ref.) | USA | Fiebig V | M-M | ccPBMC | AY835438 | 264 | 0.1 | >50 | 10.4 |
| SS1196.1 | 1B | B | USA | Fiebig V-VI | M-M | ccPBMC | AY835442 | 191 | 0.4 | 6.3 | 41.9 |
| ZM197 M.PB7 | 1B | C (ref.) | Zambia | Fiebig ≤VI | F-M | ucPBMC | DQ388515 | 527 | >50 | >50 | >50 |
| H029.12 | 1B | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210726 | 267 | >50 | >50 | >50 |
| 1012-11.TC21.3257 | 1B | B (transm.) | USA | Fiebig III | Unknown | Plasma | EU289184 | 559 | >50 | >50 | >50 |
| H086.8 | 1B | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210732 | 1,141 | >50 | >50 | >50 |
| Q23ENV17 | 1B | A1 | Kenya | Fiebig VI | FSW | ccPBMC | AF004885 | 428 | >50 | >50 | >50 |
| BZ167.12 | 1B | B | Brazil | Fiebig VI | Unknown | ccPBMC | AY173956 | 184 | 0.2 | >50 | >50 |
| 25711-2.4 | 1B | C | India | Fiebig III | F-M | ccPBMC | EF117272 | 1,307 | >50 | >50 | >50 |
| ZM109F.PB4 | 1B | C (ref.) | Zambia | Fiebig ≤VI | M-F | ucPBMC | AY424138 | 1,109 | >50 | >50 | >50 |
| 25925-2.22 | 1B | C | India | Fiebig III | F-M | ccPBMC | EF117273 | 909 | >50 | >50 | >50 |
| 1056-10.TA11.1826 | 1B | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289186 | 522 | >50 | >50 | >50 |
| 16845-2.22 | 2 | C | India | Fiebig V | M-F | ccPBMC | EF117269 | 678 | >50 | >50 | >50 |
| Du156.12 | 2 | C | South Africa | Fiebig ≤IV | FSW | ccPBMC | DQ411852 | 770 | >50 | >50 | >50 |
| TRO.11 | 2 | B (ref.) | Italy | Fiebig III | M-M | ccPBMC | AY835445 | 705 | >50 | >50 | >50 |
| 26191-2.48 | 2 | C | India | Fiebig III | F-M | ccPBMC | EF117274 | 2,439 | >50 | >50 | >50 |
| SC422661.8 | 2 | B (ref.) | Trinidad and Tobago | Fiebig IV | F-M | Plasma | AY835441 | 662 | >50 | >50 | >50 |
| WITO4160.33 | 2 | B (ref.) | USA | Fiebig II | F-M | Plasma | AY835451 | 764 | >50 | >50 | >50 |
| 0013095-2.11 | 2 | C | India | Fiebig IV | M-F | ccPBMC | EF117267 | 400 | >50 | >50 | >50 |
| REJO4541.67 | 2 | B (ref.) | USA | Fiebig II | F-M | Plasma | AY835449 | 324 | >50 | >50 | >50 |
| ZM135 M.PL10a | 2 | C | Zambia | Fiebig ≤VI | F-M | Plasma | AY424079 | 616 | >50 | >50 | >50 |
| 700010040.C9.4520 | 2 | B (transm.) | USA | Fiebig V | M-M | Plasma | EU289193 | 812 | >50 | >50 | >50 |
| Du123.6 | 2 | C | South Africa | Fiebig VI | FSW | ccPBMC | DQ411850 | 991 | >50 | >50 | >50 |
| ZM249 M.PL1 | 2 | C (ref.) | Zambia | Fiebig II | F-M | Plasma | DQ388514 | 881 | >50 | >50 | >50 |
| 266-60 | 2 | 02_AG | Cameroon | Fiebig VI | Unknown | ucPBMC | EU513193 | 1,179 | >50 | >50 | >50 |
| 16936-2.21 | 2 | C | India | Fiebig III | F-M | ccPBMC | EF117270 | 829 | >50 | >50 | >50 |
| Q842ENVd12 | 2 | A1 | Kenya | Acute/early | FSW | ucPBMC | AF407160 | 425 | >50 | >50 | >50 |
| SC05.8C11.2344 | 2 | B (transm.) | Trinidad and Tobago | Fiebig II | F-M | Plasma | EU289200 | 577 | >50 | >50 | >50 |
| PRB926-04.A9.4237 | 2 | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289197 | 318 | >50 | >50 | >50 |
| Q259ENVd2.17 | 2 | A1 | Kenya | Acute/early | FSW | ucPBMC | AF407152 | 695 | >50 | >50 | >50 |
| 255-34 | 2 | 02_AG | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513184 | 1,137 | >50 | >50 | >50 |
| H022.7 | 2 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210725 | >2,500 | >50 | >50 | >50 |
| Q769ENVd22 | 2 | A1 | Kenya | Acute/early | FSW | ucPBMC | AF407158 | 658 | >50 | >50 | >50 |
| 1006-11.C3.1601 | 2 | B (transm.) | USA | Fiebig III | Unknown | Plasma | EU289183 | 622 | >50 | >50 | >50 |
| 280-5 | 2 | 02A1U | Cameroon | Fiebig VI | Unknown | ucPBMC | EU513183 | 639 | >50 | >50 | >50 |
| CAP210.2.00.E8 | 2 | C (ref.) | South Africa | Fiebig IV | FSW | Plasma | DQ435683 | 913 | >50 | >50 | >50 |
| WEAU-d15.410.787 | 2 | B (transm.) | USA | Fiebig II | M-M | Plasma | EU289202 | 616 | >50 | >50 | >50 |
| 263-8 | 2 | A1U | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513182 | 611 | >50 | >50 | >50 |
| 0330.v4.c3 | 2 | A | Tanzania | Fiebig V/VI | M-F | Plasma | NA | 845 | >50 | >50 | >50 |
| AC10.0.29 | 2 | B (ref.) | USA | Fiebig III | M-M | ccPBMC | AY835446 | 659 | >50 | >50 | >50 |
| 3415.v1.c1 | 2 | A | Tanzania | Fiebig V/VI | F-M | Plasma | NA | 688 | >50 | >50 | >50 |
| 6240.08.TA5.4622 | 2 | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289190 | 1224 | >50 | >50 | >50 |
| 252-7 | 2 | U | Cameroon | Fiebig VI | Unknown | ucPBMC | EU513190 | >2,500 | >50 | >50 | >50 |
| CH110.2 | 2 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117257 | 379 | >50 | >50 | >50 |
| 6244.13.B5.4567 | 2 | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289191 | 664 | >50 | >50 | >50 |
| Q168ENVa2 | 2 | A1 | Kenya | Acute/early | FSW | ucPBMC | AF407148 | 926 | >50 | >50 | >50 |
| 235-47 | 2 | 02_AG | Cameroon | Fiebig VI | Unknown | ucPBMC | EU513195 | 1,112 | >50 | >50 | >50 |
| 62357.14.D3.4589 | 2 | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289189 | 730 | >50 | >50 | >50 |
| CAAN5342.A2 | 2 | B (ref.) | USA | Fiebig ≤VI | M-M | Plasma | AY835452 | 1,134 | >50 | >50 | >50 |
| H080.23 | 2 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210735 | 1,506 | >50 | >50 | >50 |
| Du172.17 | 2 | C | South Africa | Fiebig VI | FSW | ccPBMC | DQ411853 | 1,216 | >50 | >50 | >50 |
| Du151.2 | 2 | C | South Africa | Fiebig V | FSW | ccPBMC | DQ411851 | 1,091 | >50 | >50 | >50 |
| ZM233 M.PB6 | 2 | C (ref.) | Zambia | Fiebig ≤VI | F-M | ucPBMC | DQ388517 | 734 | >50 | >50 | >50 |
| CAP45.2.00.G3 | 2 | C (ref.) | South Africa | Fiebig IV | FSW | Plasma | DQ435682 | 1,813 | >50 | >50 | >50 |
| ZM214 M.PL15 | 2 | C (ref.) | Zambia | Fiebig ≤VI | F-M | Plasma | DQ388516 | >2,500 | >50 | >50 | >50 |
| 0439.v5.c1 | 2 | A | Tanzania | Fiebig V/VI | M-F | Plasma | NA | 1,283 | >50 | >50 | >50 |
| 269-12 | 2 | 02U | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513194 | 1,706 | >50 | >50 | >50 |
| 9021.14.B2.4571 | 2 | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289196 | 585 | >50 | >50 | >50 |
| H030.7 | 2 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210727 | 2,165 | >50 | >50 | >50 |
| 3718.v3.c11 | 2 | A | Tanzania | Fiebig I/II | M-F | Plasma | NA | >2,500 | >50 | >50 | >50 |
| 0260.v5.c1 | 2 | A | Tanzania | Fiebig V/VI | F-M | Plasma | NA | 623 | >50 | >50 | >50 |
| 247-23 | 2 | DU | Cameroon | Not recorded | Unknown | ucPBMC | EU683891 | 815 | >50 | >50 | >50 |
| QH0692.42 | 2 | B (ref.) | Trinidad and Tobago | Fiebig V | F-M | ccPBMC | AY835439 | 1,812 | 39.3 | >50 | >50 |
| THRO4156.18 | 2 | B (ref.) | USA | Fiebig II | M-M | Plasma | AY835448 | 841 | >50 | 32.1 | 40.3 |
| 3365.v2.c20 | 2 | A | Tanzania | Fiebig V/VI | M-F | Plasma | NA | 1361 | >50 | >50 | >50 |
| ZM53 M.PB12 | 2 | C (ref.) | Zambia | Fiebig ≤VI | F-M | ucPBMC | AY423984 | 1,903 | >50 | >50 | >50 |
| 1054-07.TC4.1499 | 2 | B (transm.) | USA | Fiebig II | Unknown | Plasma | EU289185 | 1157 | >50 | >50 | >50 |
| 16055-2.3 | 2 | C | India | Fiebig II | F-M | ccPBMC | EF117268 | >2,500 | >50 | >50 | >50 |
| RHPA4259.7 | 2 | B (ref.) | USA | Fiebig ≤V | M-F | Plasma | AY835447 | 544 | 48.9 | >50 | >50 |
| CH181.12 | 2 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117259 | 1,534 | >50 | >50 | >50 |
| 250-4 | 2 | 02_AG | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513189 | 1,072 | >50 | >50 | >50 |
| 001428-2.42 | 2 | C | India | Fiebig IV | M-F | ccPBMC | EF117266 | 660 | >50 | >50 | >50 |
| Du422.1 | 2 | C (ref.) | South Africa | Fiebig V | FSW | ccPBMC | DQ411854 | 949 | >50 | >50 | >50 |
| CH064.20 | 2 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117254 | 1,202 | >50 | >50 | >50 |
| H031.7 | 2 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210728 | 775 | 0.6 | >50 | >50 |
| CH111.8 | 2 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117258 | 2,429 | >50 | >50 | >50 |
| CH119.10 | 2 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117261 | 2,236 | >50 | >50 | >50 |
| Q461ENVe2 | 2 | A1 | Kenya | Acute/early | FSW | ucPBMC | AF407156 | >2,500 | >50 | >50 | >50 |
| 211-9 | 2 | 02_AG | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513187 | 2,241 | >50 | >50 | >50 |
| CH117.4 | 2 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117262 | 836 | >50 | >50 | >50 |
| H035.18 | 3 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210729 | >2,500 | >50 | >50 | >50 |
| 928-28 | 2 | 02_AG | Cote d'Ivoire | Acute/early | FSW | ccPBMC | EU513199 | >2,500 | >50 | >50 | >50 |
| CH070.1 | 3 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117255 | >2,500 | >50 | >50 | >50 |
| CH038.12 | 3 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF042692 | 1,219 | >50 | >50 | >50 |
| CH114.8 | 3 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117264 | 1,595 | >50 | >50 | >50 |
| H061.14 | 3 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210730 | 562 | 23.4 | >50 | >50 |
| 253-11 | 3 | CRF02_AG | Cameroon | Fiebig VI | Unknown | ucPBMC | EU513191 | >2,500 | >50 | >50 | >50 |
| 257-31 | 3 | 02_AG | Cameroon | Acute/early | Unknown | ccPBMC | EU513185 | >2,500 | >50 | >50 | >50 |
| PVO.4 | 3 | B (ref.) | Italy | Fiebig III | M-M | ccPBMC | AY835444 | 1,277 | >50 | >50 | >50 |
| TRJO4551.58 | 3 | B (ref.) | USA | Fiebig II | M-M | Plasma | AY835450 | 1,221 | >50 | >50 | >50 |
| H078.14 | 3 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210733 | 1,702 | >50 | >50 | >50 |
| 278-50 | 3 | 02A1U | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513198 | >2,500 | >50 | >50 | >50 |
| H079.2 | 3 | B | Peru | Fiebig VI | Sexual | ccPBMC | EF210731 | 1,445 | >50 | >50 | >50 |
| CH115.12 | 3 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117263 | >2,500 | >50 | >50 | >50 |
| 33-7 | 3 | 02A1U | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513186 | 1,900 | >50 | >50 | >50 |
| CH120.6 | 3 | 07_BC | China | Fiebig VI | IDU | ccPBMC | EF117260 | >2,500 | >50 | >50 | >50 |
| 251-18 | 3 | 02_AG | Cameroon | Fiebig VI | Unknown | ccPBMC | EU513196 | >2,500 | >50 | >50 | >50 |
a
HIV-1 isolates are ordered by neutralization sensitivity as determined by average log10 ID50 titers.
b
Subtypes from LANL database. ref., standard reference panel viruses; transm., transmitted/founder viruses.
c
Fiebig stage as previously defined (13).
d
M-F, male-to-female; F-M, female-to-male; M-M, male-to-male; FSW, female sex worker; IDU, intravenous drug use.
e
ccPBMC, cocultured PBMC; ucPBMC, uncultured PBMC; CSF, cerebrospinal fluid.
f
NA, not available.
g
Values are the concentration (μg/ml) at which RLU were reduced by 50% compared to the level in virus control wells. Data in bold indicate positive neutralization.