FIG. 1.

Vesicular stomatitis virus causes lethal neurotoxicity. (A) VSV-Luc localizes to and replicates in the brains of mice. Control Opti-MEM carrier or 1 × 10⁴ VSV-Luc particles were injected intracranially into SCID mice and imaged for luminescence at 7 days postinoculation. (B) H&E-stained section of brain in Opti-MEM-injected control mouse, shown at original magnification of ×400. (C) H&E-stained section of brain of VSV-Luc-injected mouse, shown at original magnification of ×400. Black arrows represent apoptosing neurons. (D) LD₅₀ determination with VSV-Luc administered intracranially. Kaplan-Meier survival curves of dose escalation in SCID mice are shown.