FIG. 5.

Viral replication of miRT VSVs is restricted in primary brain cells. (A) Cell viability, as determined by MTT assay, at 24 h post-viral infection at an MOI of 0.5 with recombinant miRNA-targeted VSVs in primary astrocytes. (B) Viral titers collected at 24 h postinfection from supernatants of cells infected with miRT VSVs in primary astrocytes (representative of three repeat experiments). (C) miRNA abundance in primary astrocytes, as determined by TaqMan miRNA expression analysis. Fold increases in miR-124, miR-125, or miR-206 over corresponding miRNAs in HeLa cells (per 5 ng small RNA) are shown. *, P < 0.001.