TABLE 3.
Titers of virus in tissues after intranasal inoculation with mutant NS4B.VGIv or the parental strain, BICv
| Virus (dpi) | Viral titera (TCID50/ml) in: |
Virus detectionb | |||||
|---|---|---|---|---|---|---|---|
| Tonsils | MLN | RFLN | Spleen | Kidneys | Blood | ||
| NS4B.VGIv | |||||||
| 1 | Neg | Neg | Neg | Neg | Neg | Neg | Neg |
| 2 | Neg | Neg | Neg | Neg | Neg | Neg | Neg |
| 3 | Neg | 1.97 | 2.1 | Neg | Neg | Neg | Neg |
| 4 | Neg | 1.97 | 2.2 | Neg | Neg | Neg | + |
| 7 | 3.2 | Neg | 3.2 | Neg | Neg | Neg | ++ |
| 9 | Neg | Neg | Neg | Neg | Neg | Neg | ND |
| BICv | |||||||
| 1 | Neg | Neg | Neg | Neg | Neg | Neg | Neg |
| 2 | 1.97 | 2.3 | Neg | Neg | Neg | Neg | Neg |
| 3 | 3.8 | 3.8 | 2.9 | 2.3 | Neg | 2.0 | +++ |
| 4 | 4.2 | 3.6 | 5.0 | 3.8 | 2.8 | 4.2 | +++ |
| 7 | 5.0 | 4.5 | 4.8 | 5.6 | 2.6 | 5.8 | +++ |
| 9 | 5 | 5.1 | 4.6 | 4.6 | 4.1 | 6.5 | ND |
a
Neg, viral titer of ≤1.8 TICD50/ml. MLN, mandibular lymph nodes; RFLN, retropharyngeal lymph nodes.
b
Immunofluorescence was performed on tonsil tissues obtained from one animal per time point using anti-E2 MAb WH303. Neg, negative. Positive reactivity was estimated from low (+) to strong (+++). ND, not determined.