FIG. 2.
Replication kinetics of viruses with the H7 HAs in MDCK cells. MDCK cells were inoculated at an MOI of 0.01 TCID50/cell with the FC (•), FC-CC HA (▴), FC-CC HA I13S (□), FC-CC HA A143T (▵), and FC-CC HA K416R (○) viruses. Supernatants were harvested at 6, 12, 24, and 48 h after inoculation and titrated by end point dilution in MDCK cells. Geometric mean titers were calculated from two independent experiments; the cutoff value was used for negative samples. Error bars indicate standard deviations. The cutoff value of the virus titration assay is indicated by a dotted line.