FIG. 7.

Mapping the 5′ ends of RTA-inducible transcripts. Poly(A)⁺ RNAs from induced (+) and uninduced (−) rKSHV.219 Vero cells were annealed to ³²P-labeled antisense oligonucleotides specific to ORF70 (lanes 1 and 2), ORF19 (lanes 7 and 8), or ORF47 (lanes 13 and 14). Primers were extended using reverse transcriptase, and the denatured products were resolved in an 8% sequencing gel. The sizes of the extension products were determined using radiolabeled molecular weight markers and a sequencing ladder generated with the same labeled primer and a subcloned fragment of KSHV genomic DNA as the sequencing template. Extension products that coincide with end points detected by 5′-RACE are indicated with lollipops.