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. 2009 Nov 11;84(3):1289–1301. doi: 10.1128/JVI.01281-09

FIG. 2.

FIG. 2.

Presence of inflammatory cells in the lungs of mice following SARS-CoV infection. (A) FACS analysis of inflammatory cells (CD11c+ PDCA1+ for pDC, CD3− DX5+ for NK, CD3+ DX5+ for NKT, CD11b+ F4/80+ for macrophages, and CD11c− Gr1+ for neutrophils) in mock-infected and SARS-CoV-infected lungs. Cells for analysis are gated on a CD45+ population. (B) Total number of inflammatory cells isolated from the lungs on the indicated day p.i. Data are the means ± SEMs for three to five mice analyzed at each time point. Results from one representative experiment out of three are shown. *, P < 0.05; **, P < 0.01 (for the comparison between mock-and SARS-CoV-infected groups) by Student's t test. (C) FACS analysis of IFN-γ-producing CD8+ and CD4+ T cells isolated from the lungs of SARS-CoV-infected mice on day 7 p.i., with or without ex vivo stimulation with PMA/ionomycin or SARS-CoV S or N peptides. Cells for analysis are gated on a CD3+ population. (D) Total number of CD8+ and CD4+ T cells producing IFN-γ in the lungs of mock- or SARS-CoV-infected mice, with or without ex vivo stimulation with PMA/ionomycin or SARS-CoV S or N peptides. Data are from pools of cells from four mice analyzed at day 7 p.i. Results from a representative experiment out of two is shown.