FIG. 4.

pSer¹⁷⁷-SHDAg interacted preferentially with RNAP IIO in vivo. Three days after transfection with 2AGwt, 293T cells were treated with 200 μM DRB (lanes 3, 9, and 10) or 0.1 μg/ml of ActD (lanes 4, 11, and 12) for 2 h. Total cellular proteins were extracted and then immunoprecipitated with rabbit anti-HDAg (α-δ; lanes 5, 7, 9, and 11) or anti-pSer¹⁷⁷-HDAg (α-pδ; lanes 6, 8, 10, and 12) antibody. Bound RNAP II and SHDAg were detected with anti-RNAP II, anti-HDAg, or anti-pSer¹⁷⁷-HDAg antibody. Inputs from transfected or mock-transfected cells are indicated.