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. 2009 Nov 18;84(3):1397–1405. doi: 10.1128/JVI.01721-09

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FIG. 2. — In vitro pulldown assay to assess the interaction between individual recombinant viral proteins and recombinant tegument protein pUL48. Lysates from HeLa cells expressing recombinant myc-tagged pUL19UD (amino acids 451 to 1054), pUL36N (amino acids 1 to 767), pUL38, pUL38C (amino acids 91 to 456), and kinesinC (amino acids 814 to 963) were added to GST or GST-tagged pUL48N (amino acids 1 to 411) immobilized on glutathione-Sepharose beads. Interacting complexes were subsequently eluted and separated by SDS-PAGE (12%). (A) Immunoblot with monoclonal anti-myc antibody shows the presence of myc-tagged pUL19UD and pUL38 coeluting with GST-tagged pUL48N. The presence of each expressed myc-tagged capsid protein in HeLa cell lysates was also confirmed. (B) Coomassie blue stain gel confirming presence of GST-tagged proteins eluted from glutathione-Sepharose beads. (C) Immunoblot with monoclonal anti-myc antibody shows the presence of myc-tagged pUL36N coeluting with GST-tagged pUL48N. The presence of each expressed myc-tagged protein in HeLa cell lysates was also confirmed. (D) Coomassie blue stain gel confirming presence of GST-tagged proteins eluted from glutathione-Sepharose beads.