Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Nov 18;84(3):1397–1405. doi: 10.1128/JVI.01721-09

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010, American Society for Microbiology

PMC Copyright notice

FIG. 3. — Pulldown assay to assess interaction of in vitro-assembled capsids with recombinant tegument protein pUL48. In vitro-assembled capsids were added to GST, GST-tagged pUL48N (amino acids 1 to 411), or GST-tagged DYNLT3 (dynein light chain rp3) immobilized on glutathione-Sepharose beads. Interacting complexes were subsequently eluted and separated by SDS-PAGE (12%). (A) Immunoblot with monoclonal anti-pUL19 antibody as a marker for capsids plus pUL35 (C) and capsids minus pUL35 (C−) shows the presence of pUL35 containing capsids coeluting with GST-tagged DYNLT3 but not GST or GST-tagged pUL48N. (B) Coomassie blue stain gel confirming presence of GST-tagged proteins eluted from glutathione-Sepharose beads and also equivalent amounts of input C and C− capsids. The position of capsid proteins in the input capsid lanes are indicated.