Skip to main content
. 2009 Nov 18;84(3):1397–1405. doi: 10.1128/JVI.01721-09

FIG. 4.

FIG. 4.

Consequence of the loss of pUL35, pUL36, or pUL37 on the addition of pUL48 to C capsids. C capsids were purified from Vero cell lysates harvested after at least 24 h postinfection with either HSV-1 parental strain 17, pUL35-minus (dmVP26-minus), pUL36-minus (ARΔUL36), or pUL37-minus (FRΔUL37) viruses. (A) Immunoblot identifying constituents of purified C capsids with mock-infected and HSV-1 parental strain 17-infected cell lysates as controls for antibody specificity. Primary antibodies to HSV-1 proteins included mouse monoclonal against pUL19, and rabbit polyclonal antibodies against pUL37, pUL48, and gD. An additional rabbit polyclonal antibody raised against purified HSV-1 nuclear C capsids (PTNC) was used to detect tegument protein pUL36, as well as the capsid proteins pUL18 and pUL35. (B) Agarose gel stained with ethidium bromide verifying the presence of viral genomic DNA in isolated C capsids. DNA was extracted from isolated capsids and amplified by using specific primers to the gene encoding HSV-1 tegument protein pUS10 (939 bp). The positive control contained as DNA template the construct displayTarget/pUS10, while the negative control contained no DNA template.