TABLE 1.
Human Xpr1 expression in 208F cells increases their susceptibility to XMRV infectiona
| Virus | Expt | Titer (AP+ FFU/ml) of LAPSN vector on cells |
Ratio | XMRV ratio normalized to 10A1 ratio | |
|---|---|---|---|---|---|
| 208F/hXpr1 | 208F | ||||
| XMRV+LAPSN | 1 | 4.55 × 106 | 2.90 × 106 | 1.57 | 1.27 |
| 2 | 2.54 × 106 | 1.90 × 106 | 1.34 | 1.34 | |
| 3 | 4.16 × 106 | 3.09 × 106 | 1.35 | 1.42 | |
| 10A1+LAPSN | 1 | 1.68 × 107 | 1.35 × 107 | 1.24 | |
| 2 | 7.40 × 106 | 7.40 × 106 | 1.00 | ||
| 3 | 1.23 × 107 | 1.30 × 107 | 0.95 | ||
a
Cells were seeded at 5×104 cells per well in 6-well plates, were infected the next day with appropriate dilutions of XMRV+LAPSN virus harvested from HTX/LAPSN+XMRV cells (XMRV was from 22Rv1 cells) or 10A1+LAPSN virus from Mus dunni/LAPSN+10A1 cells (21), and were stained for AP 2 days after infection. Results are from three independent experiments.