. 2009 Dec 2;84(4):1828–1837. doi: 10.1128/JVI.01890-09

TABLE 3.

Mutations detected after cloning of RT-PCR or PCR product of infecting RNA2 vector and control templates

RT-PCR or PCR template	No. of clones with result/total no. of clones				Frequency of point mutation^d
RT-PCR or PCR template	Without nucleotide change	Without amino acid change	With deletion mutation	With point mutation(s)^c	Frequency of point mutation^d
Total RNA at 72 hpi^a	14/23	16/23	2/23	7/23 (5/23)	8/16,422
Inoculated RNA transcript^a	14/22	17/22	0/22	8/22 (5/22)	11/15,708
Template cDNA used for transcription^b	10/24	14/24	0/24	14/24 (10/24)	24/17,136

RT-PCR product of total RNA at 72 hpi or inoculated RNA transcript.

PCR product of template DNA used for transcription.

Numbers of clones with nucleotide substitution(s) or single-nucleotide insertion(s) are shown. Numbers in parentheses show clones with amino acid changes.

Number of total point mutations/total number of sequenced nucleotides.