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. 2009 Dec 2;84(4):2110–2121. doi: 10.1128/JVI.02007-09

FIG. 2.

FIG. 2.

Characterization of recombinant viruses. (A to C) Immunoblots of electrophoretically separated lysates from Vero cells infected with wild-type HSV-1(F) (lane 1), YK651 (Venus-UL12) (lane 2), and YK653 (Flag-UL12) (lane 3). Infected cells were harvested 18 h postinfection and analyzed by immunoblotting with antibody to UL12 (A), GFP (B), and Flag (C). (D and E) Vero cells were infected at an MOI of 5 (D) or 0.01 (E) with wild-type HSV-1(F), YK651 (Venus-UL12), or YK653 (Flag-UL12). Total virus from cell culture supernatants and infected cells harvested at the indicated times were assayed on Vero cells.