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. 2009 Dec 2;84(4):2110–2121. doi: 10.1128/JVI.02007-09

FIG. 9.

FIG. 9.

Confocal microscopy of infected nucleolin knock-down cells. (A) Vero-shNuc-4, Vero-shNuc-5, and Vero-shGFP cells infected with wild-type HSV-1(F) for 12 h were analyzed by immunoblotting with antibodies to nucleolin, UL12, and α-tubulin. (B) Quantitation of the amount of nucleolin protein relative to the amount of α-tubulin shown in panel A. The shGFP value was normalized to 100. (C) Vero (a and b), Vero-shGFP (c and d), Vero-shNuc-5 (e and f), and Vero-shNuc-4 (g and h) cells were infected with YK601 (VenusA206K-VP26) for 12 h and then examined by confocal micros- copy. Confocal Z-sections were acquired through the entire thickness of the cells. A confocal Z-stacked fluorescence image derived from the Z-sections (a, c, e, and g) and DIC (b, d, f, and h) images is shown. Insets show magnified images of the boxed areas. Scale bar, 500 nm. (D) Quantitation of virus particles in the cytoplasm of infected cells from the data in panel C.