FIG. 1.

After RV infection, DCs in PPs increase their absolute number and migrate to the SED. Groups of four mice were inoculated orally with 10⁴ FFU of the murine RV EDIM_wt. Total cells from PPs of the small intestine were obtained from RV EDIM_wt-infected mice after 0 (control mice), 24 and 48 h p.i.; stained with rat anti-mouse CD11c and CCR6 MAbs; and analyzed by flow cytometry. (A) The absolute number of DCs was calculated by multiplying the percentage of DCs by the total cell number per PP. Shown are cells from noninfected mice (black bars) and from RV-infected mice 24 (gray bars) and 48 h p.i. (light gray bars). Bars represent the standard deviations. An asterisk indicates significant difference with respect to the control (P < 0.05, Student's t test). (B) PP from jejunum were obtained from mice at 0, 12, 24, and 48 h p.i. and cryopreserved. The frozen sections of the PPs were stained with the MAb hamster anti-mouse CD11c (clone N418) and developed with a secondary goat anti-hamster IgG Ab coupled to peroxidase using 3-amino-9-ethylcarbazole chromogen substrate. (a, b, c) Histological analysis of CD11c⁺ cells in PPs of noninfected mice. (d, e, f) CD11c⁺ cells in PPs at 12 h p.i. (g, h, i) CD11c⁺ cells in PPs at 24 h p.i. (j, k, l) CD11c⁺ cells in PPs at 48 h p.i. Arrows indicate CD11c⁺ cells. L, intestinal lumen; SED, subepithelial dome. The experiment has been repeated with similar results with at least 30 PPs of jejunum and ileum. (C) Histogram of CCR6 MFI of CD11c⁺ cells from PPs. Isotype control (light gray histogram), cells from noninfected mice (dotted line), and cells from RV-infected mice at 48 h p.i. (solid line) are shown. The histogram is respresentative of three independent experiments.