FIG. 4.

Inhibition of Raf-1 impairs MVM nuclear capsid assembly. Synchronous cultures infected with MVMp were released from the arrest and treated (+) or not (−) with Radicicol (Rad) at 14 hpi. (A) Accumulation of Raf-1 and of VP proteins along the infection of NB324K cells shown by Western blots. (B to D) Confocal analysis of the subcellular distribution of VP proteins and MVM capsid in normal and Raf-1-inhibited (+Rad) cells at 20 hpi. (B) NB324K transformed human fibroblasts; (C) A9 mouse fibroblasts; (D) U373 human glioma.