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NS1 and NS2 synthesis in influenza C virus-infected and NS gene-transfected cells. COS-1 cells mock infected (lane mock), infected with C/Yamagata/1/88 (Infect lanes) at an MOI of 10 PFU/cell, or transfected with pME18S-WTNS (WTNS lanes) were labeled with [35S]methionine and immunoprecipitated with anti-GST/NS1 serum (αNS1 lanes) or anti-GST/NS2 serum (αNS2 lanes). The resulting immunoprecipitates were analyzed by SDS-PAGE, followed by fluorography.
