TABLE 1.
Sample collections tested by using the degenerate primers for replication-associated proteins
| Sample collection | Collection site(s) (country) | Specimen type | No. of specimens | No. of specimens (%) |
|||
|---|---|---|---|---|---|---|---|
| Pan-Rep PCR-positive | With cyclovirus | With circovirus | With other Rep proteins | ||||
| South Asian children with | Pakistan, Afghanistan | Human stool | 57 (diseased) | 12 (21.1) | 9 (15.8) | 0 (0.0) | 3 (5.3) |
| AFP and healthy | 9 (contact) | 3 (33.3) | 3 (33.3) | 0 (0.0) | 0 (0.0) | ||
| contact and control children | 41 (control) | 8 (19.5) | 7 (17.1) | 0 (0.0) | 1 (2.4) | ||
| Minnesotans with | United States | Human stool | 140 (diseased) | 7 (5.0) | 0 (0.0) | 7 (5.0) | 0 (0.0) |
| gastroenteritis and healthy controls | 107 (control) | 6 (5.6) | 0 (0.0) | 6 (5.6) | 0 (0.0) | ||
| Nigerian children with AFP | Nigeria | Human stool | 96 | 18 (18.8) | 9 (9.4) | 2 (2.1) | 7 (7.3) |
| Tunisian children with | Tunisia | Human stool | 96 (diseased) | 7 (7.3) | 7 (7.3) | 0 (0.0) | 0 (0.0) |
| AFP and healthy contact children | 96 (contact) | 9 (9.4) | 7 (7.3) | 1 (1.0) | 1 (1.0) | ||
| African chimpanzees | Middle African countriesa | Chimpanzee stool | 44 | 9 (20.5)a | 6 (13.6) | 3 (6.8) | 1 (2.3) |
| U.S. blood donors | United States | Human plasma | 96 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
| African bush hunters | Africa | Human plasma | 113 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
| U.S. pork products | United States | Pork | 13 | 9 (69.2) | 0 (0.0) | 9 (69.2) | 0 (0.0) |
| Pakistani meat | Pakistan | Chicken | 13 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
| Beef | 26 | 5 (19.2) | 4 (15.4) | 1 (3.8) | 0 (0.0) | ||
| Goat | 18 | 7 (38.9) | 7 (38.9) | 0 (0.0) | 0 (0.0) | ||
| Nigerian meat | Nigeria | Chicken | 40 | 30 (75.0) | 22 (55.0) | 8 (20.0) | 0 (0.0) |
| Beef | 25 | 3 (12.0) | 3 (12.0) | 0 (0.0) | 0 (0.0) | ||
| Camel | 27 | 3 (11.1) | 3 (11.1) | 0 (0.0) | 0 (0.0) | ||
| Goat | 26 | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | ||
| Sheep | 29 | 1 (3.4) | 1 (3.4) | 0 (0.0) | 0 (0.0) | ||
| Total | 1,112 | 137 | 88 | 37 | 13 | ||
a
Specimens were collected from Tanzania, Cameroon, Uganda, Rwanda, Central Africa Republic, Republic of the Congo, and the Democratic Republic of the Congo. Two different Rep sequences were obtained from one chimpanzee specimen by subcloning.